Bioprinting Living Biofilms through Optogenetic Manipulation.
blue
red
BlrP1
BphS
P. aeruginosa
Control of cell-cell / cell-material interactions
Immediate control of second messengers
Multichromatic
Abstract:
In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living bacteria to autonomically form patterned biofilms following prescribed illumination. The results showed that through optogenetic manipulation, patterned bacterial communities with high spatial resolution (approximately 10 μm) could be constructed in 6 h. Thus, optogenetic manipulation greatly increases the range of available bioprinting techniques.
